Using a CRISPR-KO library on a migration screen

DISCLAIMER I assume that you have working knowledge of vectors (plasmids) used in molecular biology, and know what an RNA or sgRNA is. Anything else is not important or will be explained.

My master’s thesis will be published soon on the NUS scholar’s bank. While I’m proud of what I’ve managed to do, the wet lab side of the work almost broke me! For instance, an image in the thesis showing a western blot of DLG5 Knock-Out (KO) took almost 3 months of work. The problem was not, as one would think, that DLG5 is >300 kDa, but that the antibodies were fussy… >two days incubation in primary antibodies was the solution.