Generating Custom Raspberry-Pi Images
I’ve been working on a number of projects involving my raspberry pi; DSLR timelapse, a pi running dedicated stocks and notifications systems, and a media server. I have a silly approach of solving any critical errors by flashing a new raspbian image to the SD card and continuing as if the error was never made. The approach can be heavily criticized, but I’m not invested in immediately working out the errors; it’s usually something obscure. 90% of the time, some critical software was just installed the wrong way. I’ve been looking for easy ways to set up my raspbian images from the get-go and found what I think is the best approach: Pi-gen.
Digitizing collections
I’ve begun creating a media server lately. Something that I never thought I would attempt. With the number of relatively comprehensive streaming services, a media server did not make much sense. Not to mention my ptsd thinking back to my teenage years of terrible data-handling. I would create inumerable backups with minute differences, making it impossible to realize which one was the proper backup when needed. I’m still surprised today by some backup hidden deep in my file systems. I imagine I’ve learnt something from my last few years with linux, where I’ve learned rules to file managment.
Tidymodels and Palmers Penguins
Oddly enough, I’ve been hearing a lot about tidymodels code week, from reddit to youtube.
Getting Emacs and Julia to Work
Julia and Emacs
Julia is a hot upcoming language. I see nothing but support for it online, and many benchmarks showing a notable improvements over Python or R. The main reason why it is not already rivaling Python or R is the framework and packages. The language is still new, and does not have a mature framework. Even though it is now on the stable v1.4, there are features I’ve seen described as as ‘in development’. The packages are also maturing, and I can find many equivalent packages to those I use a biologist. However, I will admit a certain level of distrust until I understand Julia better.
New-Years Resolution
On 31st Dec, 2019, I was sitting in Kolkata, India, on the lawn of the Kolkata Club. In front was a stage, musicians and DJs from bollywood. Around me were relatives and family, ready to celebrate the New Years with a big dinner and lots of musical entertainment. And for all the excitment around me, it felt like any other New Years. [[https://www.youtube.com/watch?v=eAFnby2184o][John Oliver]] said it well when he described New Years as ‘/The Worst!/’. My track record hasn’t been great with New Years. For all the hype we put on New Year, New Me, we end up falling back into the same habits. So ironically, I came up with a goal on New Years to spite to all my failed New Years resolution; to write 1-2 scripts a week for a year in any programming language.
Eink-calendar
Archived
Using a CRISPR-KO library on a migration screen
DISCLAIMER I assume that you have working knowledge of vectors (plasmids) used in molecular biology, and know what an RNA or sgRNA is. Anything else is not important or will be explained.
My master’s thesis will be published soon on the NUS scholar’s bank. While I’m proud of what I’ve managed to do, the wet lab side of the work almost broke me! For instance, an image in the thesis showing a western blot of DLG5 Knock-Out (KO) took almost 3 months of work. The problem was not, as one would think, that DLG5 is >300 kDa, but that the antibodies were fussy… >two days incubation in primary antibodies was the solution.
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About the Blog
I started this blog to figure out static web programming. It is a record of hobbies, programming, and interests. Mostly, its an effort to practice public writing.
If you want to see some of the places me and my wife see, check out our Instagram page here!
About Me: Shayonendra N. Tagore
I am an ex-scientist, moving away from the bench. As a scientist, I have been involved in Pfizer’s COVID-19 vaccine, NGS screens with CRISPR-KO, and designing parasite animal models. Lately, I’ve been involved in automation in biotech and begun my FT MBA at NYU Stern. Please feel free to reach out to me at snt.scouting879@passmail.com.